Tag
OmpT-hlyA

Part:BBa_K3093011:Design

Designed by: Xiang Lan   Group: iGEM19_ECUST_China   (2019-10-16)


HlyA-tag+Secretion system(OmpT-cleavable linker)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1578
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1334


Design Notes

Front-primer: ggtagcgcacgacgagcaggcggtggcggttctttagcctatggaagtcaggg Rear-primer: cgcctgctcgtcgtgcgctaccgccaccgccagcaccggtggagtgacgac


Source

PCR the sequence for a composite part BBa_K1166002 devised by iGEM13_tecMonterrey team.

References